Effects of Black Jade on Osteogenic Differentiation of Adipose Derived Stem Cells under Benzopyrene

Abstract

Jade, a popular gemstone symbolizing beauty, grace, and longevity, is known to improve blood circulation; however, scientific research evidence is still lacking. The effect of black jade extract on the expression levels of apoptotic and osteogenic genes was validated using qPCR and flow cytometry. In combination with the use of a fluorescence microscope, osteogenic differentiation and the stained osteocytes count were analyzed. Under the pressure of benzo(a)pyrene, dermal cell apoptosis was accelerated and the osteogenic differentiation of adipose-derived stem cells (ASCs) was suppressed; but black jade extract counteracted the effects. Through an anti-apoptotic mechanism, the extract suppressed the expression of apoptotic proteins Bax and cytochrome C to 9 and 4.8 times, respectively, compared to that in dermal cells exposed to benzo(a)pyrene. During osteogenic differentiation of ASCs, the extract enhanced their differentiation despite being exposed to benzo(a)pyrene, and the relative levels of the osteoblast differentiation markers osteoponin, osteocalcin, and sclerostin were 1.87, 2.54, and 3.9 times higher, respectively, than those in the conditioned medium by benzo(a)pyrene. These effects of the extract indicate that black jade extract is very useful when applied as a functional biomaterial. View Full-Text

Keywords: osteogenesis; black jade; adipose-derived stem cell; fine particles; osteocyte

 

 

Department of Clinical Laboratory Sciences, College of Health Science, Korea University, Seoul 02841, Korea
Dr. JADE, 1-26, Baekbeom-ro 16-gil, Mapo-gu, Seoul 04149, Korea

Department of Philosophy of Science, SunMoon University, Asan-si 336-708, Korea

Life Together, 13, Gongdan-ro, Chuncheon-si 24232, Korea

Mitosbio, 13, Gongdan-ro, Chuncheon-si 24232, Korea

 

Appl. Sci. 2021, 11(3), 1346; https://doi.org/10.3390/app11031346

 

This study was supported by Dr. JADE Co., Ltd., LIFE TOGETER and Korea University. 

https://www.mdpi.com/2076-3417/11/3/1346/htm

 

본 발명은 옥추출물에 의한 줄기세포의 골세포 분화 방법에 관한 것으로서, 보다 상세하게는 1) 옥 추출물을 피부세포(Fibroblast)에 처리하여 활성화된 기능성 성분을 배출시키는 단계; 2) 상기 기능성 성분이 포함된 배지의 상층액을 분리하는 단계; 3) 상기 분리된 배지 상층액을 줄기세포에 처리하여 배양하여 골세포로 분화시키는 단계; 및 3) 상기 분화된 골세포를 분리하는 단계;를 포함하는 옥추출물에 의한 줄기세포의 골세포 분화 방법 및 상기 방법에 의해 제조된 골세포가 함유된 피부세포 복원 또는 관절염 치료용 조성물과 화장료 조성물에 관한 것이다. 본 발명의 골세포 분화방법을 사용할 경우 지방유래 줄기세포를 골세포로 분화시킬 수 있다. 따라서, 본 발명에 따른 골세포분화 촉진은 옥추출물의 성장기 유아의 골발육과 중장년의 골다공증 예방 등에 효과적으로 사용될 수 있으며, 본 발명에 따라 얻어진 골세포는 일반 흉터, 화상 흉터와 같은 피부질환과 골 결손부 시술 등의 골대사성 질환 등의 치료에 유용하게 사용될 수 있다.

 

The present invention relates to a method for differentiating bone cells of stem cells by a jade extract. More specifically, the present invention relates to a method for differentiating bone cells of stem cells by a jade extract and a composition for restoring skin cells or treating arthritis and a cosmetic composition containing bone cells manufactured by the method, wherein the method comprises the following steps: 1) treating the jade extract to skin cells to discharge activated functional ingredients; 2) separating a supernatant of a medium containing the functional ingredients; 3) treating the separated medium supernatant to stem cells and culturing the same to differentiate into bone cells; and 4) separating the differentiated bone cells. When using the method for differentiating bone cells, adipose-derived stem cells can be differentiated into bone cells. Therefore, the promotion of bone cell differentiation according to the present invention can be effectively used for bone development of infants in a growth period and prevention of osteoporosis in the middle-aged. In addition, bone cells obtained according to the present invention can be usefully used in the treatment of skin disease such as general scars and burn scars, and bone metabolic diseases such as bone defect part procedures.

https://patents.google.com/patent/KR102179155B1/ko

https://patents.google.com/patent/KR102179155B1/en

 

 

https://www.mdpi.com/2076-3417/11/3/1346/htm

https://patents.google.com/patent/KR102179155B1/ko

https://weekly.knou.ac.kr/articles/view.do?artcUn=2212